Tris(hydroxymethyl)aminomethane

☑ Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris was used for protein crystal growth under different pH conditions.
☑ The low ionic strength of Tris buffer can be used to form the intermediate fiber of lamin in C. elegans.
☑ Tris is also one of the main components of protein electrophoresis buffer.
☑In addition, Tris is an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
☑Trimethylaminomethane is widely used in acute metabolic and respiratory acidemia. It is an alkaline buffer and has a good buffer effect on metabolic acidosis and enzyme activity reaction.
☑Tris is often used as biological buffer, and its pH value is 6.8, 7.4, 8.0, 8.8. Its structure formula, pH value changes greatly with temperature. Generally speaking, the pH value decreases by 0.03 when the temperature increases by one degree. Tris is widely used in the preparation of buffer solution in biochemistry and molecular biology experiments. For example, both TAE and tbe buffers (used for nucleic acid dissolution) commonly used in biochemical experiments need Tris. Because it contains amino groups, it can react with aldehydes.
☑ Tris is a weak base, and its PKA is 8.1 at room temperature (25 ℃). According to the buffer theory, the effective buffer range of Tris buffer is between 7.0 and 9.2. The pH value of Tris base aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain the buffer solution with this pH value. At the same time, we should pay attention to the effect of temperature on pKa of Tris. Because Tris buffer is a weak alkaline solution, DNA will be deprotonated in such a solution to improve its solubility. People often add EDTA into Tris hydrochloric acid buffer to make "Te buffer", which is used for DNA stabilization and storage. If the acid solution of adjusting pH value is replaced by acetic acid, the "Tae buffer" (Tris / acetate / EDTA) is obtained, and the "tbe buffer" (Tris / borate / EDTA) is obtained by replacing it with boric acid. These two buffers are usually used in nucleic acid electrophoresis experiments.